Conversion of mammalian 3alpha -hydroxysteroid dehydrogenase to 20alpha -hydroxysteroid dehydrogenase using loop chimeras: Changing specificity from androgens to progestins
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Conversion of mammalian 3alpha-hydroxysteroid dehydrogenase to 20alpha-hydroxysteroid dehydrogenase using loop chimeras: changing specificity from androgens to progestins.
Hydroxysteroid dehydrogenases (HSDs) regulate the occupancy and activation of steroid hormone receptors by converting potent steroid hormones into their cognate inactive metabolites. 3alpha-HSD catalyzes the inactivation of androgens in the prostate by converting 5alpha-dihydrotestosterone to 3alpha-androstanediol, where excess 5alpha-dihydrotestosterone is implicated in prostate disease. By co...
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Rat liver 3alpha-hydroxysteroid/dihydrodiol dehydrogenase (3alpha-HSD/DD), a member of the aldo-keto reductase superfamily, inactivates circulating steroid hormones and may contribute to the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs) by oxidizing trans-dihydrodiols to reactive o-quinones with the concomitant generation of reactive oxygen species. The 3alpha-HSD/DD gene has been ...
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A 17fl-hydroxysteroid dehydrogenase activity has been demonstrated in the microsomal preparations made from the skin of newborn rats. The enzyme prefers NAD to NADP as the coenzyme and utilizes 17fi-estradiol as well as testosterone as substrates. The apparent K, values estimated for 17/?-estradiol are 4.1 X 10m6 M, for estrone 1.0 X 10d5 M, and for testosterone 4.0 X 10e6 M. The enzyme can be ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1999
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.96.20.11161